Coding

Part:BBa_K1809023:Design

Designed by: David Morgan   Group: iGEM15_WPI-Worcester   (2015-08-26)


BrAFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 328
    Illegal AgeI site found at 102
    Illegal AgeI site found at 357
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.


Source

Taken from the fish Brachyosis rostratus. Pubmed ID: 16754975

References